Recombinant Anti-Cleaved Caspase-7 antibody [EPR22840-25] KO Tested (ab256469) (2024)

Table of Contents
Product price, shipping and contact information Abpromise Overview Product name Description Host species Tested applications Species reactivity Immunogen Positive control General notes Properties Form Storage instructions Storage buffer Purity Clonality Clone number Isotype Research areas Associated products Alternative Versions Compatible Secondaries Isotype control Related Products Applications The Abpromise guarantee Target Relevance Cellular localization Database links Alternative names Images Protocols Datasheets and documents SDS download Datasheet download Certificate of Compliance References (4) Customer reviews and Q&As References

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Recombinant Anti-Cleaved Caspase-7 antibody [EPR22840-25] KO Tested (ab256469) (1)

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Overview

  • Product name

    Anti-Cleaved Caspase-7 antibody [EPR22840-25]
    See all Cleaved Caspase-7 primary antibodies

  • Description

    Rabbit monoclonal [EPR22840-25] to Cleaved Caspase-7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate, Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate, mouse and rat spleen whole cell lysate.IP: HeLa treated with 1 µM Staurosporine for 3 hours whole cell lysate .

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production

    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    See Also
    Recombinant Anti-pro Caspase-7 antibody [Y33] KO Tested (ab32067)Anti-Caspase-7 antibody [E22] KO Tested (ab32522)Anti-Caspase-7 antibody [E22] - BSA and Azide free KO Tested (ab284673)Human Cleaved Caspase-7 (Asp198) ELISA Kit (ab275900)

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

  • Recombinant Anti-Cleaved Caspase-7 antibody [EPR22840-25] KO Tested (ab256469) (2)Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22840-25

  • Isotype

    IgG

  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)

  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab256469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB

1/1000. Detects a band of approximately 22, 18 kDa (predicted molecular weight: 34 kDa).
IP

1/30.

Notes

WB
1/1000. Detects a band of approximately 22, 18 kDa (predicted molecular weight: 34 kDa).

IP
1/30.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Relevance

    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

  • Cellular localization

    Cytoplasmic

  • Database links

  • Alternative names

    • active caspase 7 antibody
    • Apoptotic protease Mch3 antibody
    • CASP 7 antibody

    see all

Images

  • Western blot - Anti-Cleaved Caspase-7 antibody [EPR22840-25] (ab256469)

    All lanes : Anti-Cleaved Caspase-7 antibody [EPR22840-25] (ab256469) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 3 : Untreated wild-type HAP1 whole cell lysate
    Lane 4 : Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 5 : Untreated caspase-7 knockout HAP1 whole cell lysate
    Lane 6 : Caspase-7 knockout HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 7 : Mouse spleen lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 18,22 kDa why is the actual band size different from the predicted?

    Exposure time: 26 seconds

    Caspase-7 is activated upon apoptotic induction. Caspase-7 p22 subunit (22 kDa) and p20 subunit (18 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 16352606). This antibody reacts with an unidentifiable protein around 48 kDa. ab256469 was shown to specifically react with Caspase-7 in wild-type HAP1 cells as signal was lost in Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. ab256469 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ instrument using the ECL technique. Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Cleaved Caspase-7 antibody [EPR22840-25] (ab256469)

    Cleaved Caspase-7 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1µM Staurosporine for 3h whole cell lysate 10µg with ab256469 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256469. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1µM Staurosporine for 3h whole cell lysate 10µg

    Lane 2: ab256469 IP in HeLa treated with 1µM Staurosporine for 3h whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256469 in HeLa treated with 1µM Staurosporine for 3h whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This antibody reacts with an unidentifiable protein around 48 kDa.

  • Anti-Cleaved Caspase-7 antibody [EPR22840-25] (ab256469)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

    Recombinant Anti-Cleaved Caspase-7 antibody [EPR22840-25] KO Tested (ab256469) (6)

  • Datasheet download

    Download

Certificate of Compliance

References (4)

Publishing research using ab256469? Please let us know so that we can cite the reference in this datasheet.

ab256469 has been referenced in 4 publications.

  • Ma H & Ma Y Dapagliflozin Inhibits Ventricular Remodeling in Heart Failure Rats by Activating Autophagy through AMPK/mTOR Pathway. Comput Math Methods Med 2022:6260202 (2022). PubMed: 36193200
  • Zhao X et al. Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway. J Ophthalmol 2021:6694050 (2021). PubMed: 33628480
  • Shen H et al. Curcumin analog B14 has high bioavailability and enhances the effect of anti-breast cancer cells in vitro and in vivo. Cancer Sci 112:815-827 (2021). PubMed: 33316116
  • Liu H et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). PubMed: 33356694

Customer reviews and Q&As

Recombinant Anti-Cleaved Caspase-7 antibody [EPR22840-25] KO Tested (ab256469) (7)

Recombinant Anti-Cleaved Caspase-7 antibody [EPR22840-25] KO Tested (ab256469) (2024)

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