Recombinant Anti-pro Caspase-7 antibody [Y33] KO Tested (ab32067) (2024)

Overview

• Product name

  Anti-pro Caspase-7 antibody [Y33]
  See all pro Caspase-7 primary antibodies

• Description

  Rabbit monoclonal [Y33] to pro Caspase-7

• Host species

  Rabbit

• Specificity

  The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.

• Tested applications

  Suitable for: WB, IHC-Pmore details
  Unsuitable for: Flow Cyt or IP

• Species reactivity

  Reacts with: Human
  

• Immunogen

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• Positive control

  • Jurkat cell lysate and human lung squamous carcinoma.

• General notes

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  For more information see here.

  See Also

  Recombinant Anti-Cleaved Caspase-7 antibody [EPR22840-25] KO Tested (ab256469)Anti-Caspase-7 antibody [E22] KO Tested (ab32522)Anti-Caspase-7 antibody [E22] - BSA and Azide free KO Tested (ab284673)Human Cleaved Caspase-7 (Asp198) ELISA Kit (ab275900)

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• Storage buffer

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

• Concentration information loading...

• Purity

  Protein A purified

• Clonality

  Monoclonal

• Clone number

  Y33

• Isotype

  IgG

• Research areas

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • Caspases

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Caspases

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Cell Death
  • Apoptosis
  • Apoptosis Markers
  • Caspases

  • Cancer
  • Cell Death
  • Apoptosis
  • Metabolism

Associated products

• Alternative Versions

  • Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)

• Isotype control

  • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

• Positive Controls

  • Jurkat whole cell lysate (ab7899)

Applications

Target

• Function

  Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
  -Gly-217' bond. Overexpression promotes programmed cell death.

• Tissue specificity

  Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

• Sequence similarities

  Belongs to the peptidase C14A family.

• Post-translational
  modifications

  Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

• Cellular localization

  Cytoplasm.

• Information by UniProt

• Database links

  • Entrez Gene: 840 Human
  • Omim: 601761 Human
  • SwissProt: P55210 Human
  • Unigene: 9216 Human

• Alternative names

  • apoptosis-related cysteine peptidase antibody
  • Apoptotic protease Mch-3 antibody
  • CASP-7 antibody

  see all

Images

Protocols

Datasheets and documents

References (2)